Mini-prep

- fill one 1,5ml tube for each test tube with bacterial culture
- centrifugation (1', 13000rpm)
- discard supernatant
- resuspend pellet in 100l 50mM Tris + 10mM EDTA
- vortex brieflyadd 200l 0.2M NaOH + 1% SDS, invert 5-10 times
- add 150l 3M potassium acetate, invert 5-10 times
- centrifugation (10', 13000rpm)
- decant approx. 400 l into a new 1.5ml tube
- add 1ml cold 98% ethanol, invert 5-10 times
- centrifugation (10', 13000rpm)
- discard supernatant, add 1ml 70% ethanol
- centrifugation (5', 13000rpm)
- discard as much supernatant as possible
- incubate at 37C with open lid for 15'
- resuspend in 40l ddH2O
- incubate at 37C (10', 550rpm)
- store at -20C
